Britain’s Kyle Edmund was knocked out of Queen’s 6-3 7-5 in the first round by Greek top seed Stefanos Tsitsipas.
Trailing 6-3 3-3 when bad weather ended play on Wednesday, Edmund was unable to turn the match around on Thursday as British interest in the singles ended.
Edmund, 24, saved six match points before the 20-year-old Greek clinched victory with a backhand down the line.
Tsitsipas returned later to beat Jeremy Chardy 4-6 7-6 (7-0) 7-6 (7-4) and secure his place in the quarter-finals.
He will face eighth seed Felix Auger-Aliassime after the 18-year-old Canadian ended the challenge of Grigor Dimitrov 6-4 6-4 and controversial Australian Nick Kyrgios 6-7 (4-7) 7-6 (7-3) 7-5.
Three first-round singles matches had to be completed at the start of play on Thursday after Tuesday’s play was washed out and only a handful of matches were completed on Wednesday.
But the highlight on day four of the Fever-Tree Championships was the return of Andy Murray after five months out following a hip operation.
He teamed up with Spain’s Feliciano Lopez to beat top doubles seeds Juan Sebastian Cabal and Robert Farah in straight sets at the Fever-Tree Championships in London.
Tsitsipas punishes Edmund
Edmund was making his competitive return after retiring from the French Open with a knee injury earlier this month.
The Yorkshireman did not look his sharpest in the first set on Wednesday as he struggled to cope with Tsitsipas’ power and precision.
Flashes of sunshine greeted them when they returned on Thursday lunchtime as Edmund looked to make a quick start.
The world number 30 was unable to convert a break point in the seventh game and it proved costly when Tsitsipas eventually ended his resistance by taking his seventh match point when a Hawk-Eye replay showed his backhand had just clipped the line.
Champion Cilic, Anderson & Wawrinka all beaten
Croatia’s defending champion and fifth seed Marin Cilic was a surprise second-round casualty, losing 6-4 6-4 to Queen’s Club debutant Diego Schwartzman.
Argentine Schwartzman, 26, secured early breaks in both sets to wrap up only his third career win on grass.
Second seed Kevin Anderson, who beat Britain’s Cameron Norrie in round one, lost in three sets to France’s Gilles Simon.
The big-serving 33-year-old South African – last year’s Wimbledon runner-up – has missed most of the season because of an elbow injury and his rustiness told as Simon ran out a 6-1 4-6 6-4 winner.
Seventh seed Stan Wawrinka was also knocked out, losing 3-6 7-5 7-6 (7-2) to 37-year-old world 191 Nicolas Mahut.
Mahut will face fellow Frenchman Simon in the next round.